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Fig. 2. Typical recording of a hyperoxic switch recording of Drosophila melanogaster. Eleven flies, total mass 9.41 mg, at 20°C and 40 ml min–1 STP. The recording begins and ends with baselines. Two water excretion events can be seen at ~20–30 min into the recording. The large dip in CO2 that follows hyperoxia is plainly visible at ~65 min. A modest drop in H2O coincides with the drop in CO2. To minimize the influence of long-term drift on our measurements, we used only the magnitude of the H2O drop immediately coinciding with the CO2 drop. Following the introduction of nitrogen, a rapid rise in both H2O and CO2 are evident. CO2 rapidly declines to near baseline levels, but H2O plateaus at an intermediate level; see text.