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Fig. 4. Action of ENpa, GSPRFFamide or NdWFamide on the ACh-induced contraction of the vasoconstrictor muscle. (A) Blockade of the nerve-evoked contraction by 10–3 mol l–1 hexamethonium. Each contraction was evoked by the stimulus train (1 ms, 1.6 V, 10 Hz) for 0.5 s every 20 s. (B) Effect of ENpa (Bi), GSPRFFamide (Bii) or NdWFamide (Biii) on the ACh-induced contraction. The contraction was evoked by perfusion of 10–6 mol l–1 ACh for 40 s as indicated by bars. Peptide was applied between arrows.