Fig. 1. Expression of capa receptor in dipteran tubules. RT-PCR of tubule cDNA templates with primers designed to the capa receptor gene from D. melanogaster (A, lane 1; product size, 1448 bp), A. aegypti (A, lane 3), A. stephensi (B, lane 1; product size, 250 bp), A. gambiae (B, lane 3; product size, 250 bp). Controls were performed using no cDNA template: D. melanogaster (A, lane 2), A. aegypti (A, lane 4), A. stephensi (B, lane 2), A. gambiae (B, lane 4). L=1 kb ladder. No PCR product was observed with A. aegypti cDNA template. In all cases, products obtained were of the sizes predicted for cDNA templates. Identical PCR products were obtained from both A. gambiae and A. stephensi. Very few A. gambiae were available for study; however, given the documented expression of the putative capa receptor in both species, the more abundant A. stephensi was used for all subsequent experiments.