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Fig. 2. The calibration if the 13C-labelled bicarbonate technique in cockatiels 20–30 min after injection. (A) There was no significant relationship if isotope elimination rate (kc) alone is used for the comparison with the metabolism (O2) measured simultaneously by indirect calorimetry (regression; y=10.6x+3.8, r2=0.11, F1,20=2.35, P=0.14) with a low r2 of 10.99%. (B) The relationship of oxygen consumption to the product of elimination rate and bicarbonate pool size was significant (regression; y=5.12x+0.3, r2=0.72, F1,20=49.54, P<0.001) with a high r2 of 72.3%.