Fig. 3. Relative abundance of MSTN1 and MSTN2 RNAs and MSTN immunoreactive protein (MIP; inset) in transgenic and control coho embryos taken prior to fertilization through hatching. Values are means ± S.E.M. (N=3). For RNA data, bars that do not share identical lowercase letters are significantly different when compared within fish group (transgenic, size-matched.) For protein data (inset), bars that do not share identical lowercase letters are significantly different when compared within fish group or within age at P0.05. The 42 kDa MIP was only detected at these three sampling ages shown, and the 14 kDa MIP was not detected in any embryo samples examined.