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Fig. 6. Effect of 10–4 mol l–1 hydrochlorothiazide on secretion rate (mean ± S.E.M.) of tubules bathed in control saline and K+-free saline. Tubules were exposed to the vehicle alone (filled symbols; 0.1% ethanol) or to the vehicle plus hydrochlorothiazide (open symbols). Arrows indicate the time of addition of hydrochlorothiazide. The number of tubules per group is indicated in parentheses.