Fig. 1. The outcome of chimerism under variable water temperature regimen. (A) Representative cases for two water temperature regimen (15°C, 25°C) are depicted. Microsatellite profiles (locus PB-41) of three Botryllus chimeras (nos. 1, 2, 5; see Table 1), 8 months following allogeneic fusions of genotypic combination A vs B. Allele sizes of genotype A (alleles 173 bp and 178 bp) and genotype B (homozygotic on allele 176 bp) are marked on the left. The soma of both 15°C chimeras is typed as AB whereas at 25°C it is typed as A. (B) Typing of three off spring from chimera no. 2 (15°C; see Table 1) and nine offspring from chimeras 3 and 4 (20°C). All offspring reveal the A genotype only. (C) Part of the amplified fragment length polymorphism (AFLP) profiles of chimerical combinations (a negative, to better depict the AFLP loci, is marked as white bands). Left two lanes: genotypes C and D; specific bands are marked with arrowheads (filled for genotype C, open for genotype D). The panel depicts an example of chimeras under 15°C, 20°C and 25°C. The two soma samples at 20°C reveal cases of mixed chimeras. Th e three soma samples at 15°C and 25°C reveal cases in which frequencies of the other partner's alleles are <20%.