Fig. 6. Specificity check of the urea transporter (UT) antibodies by absorption test. (A,B) The same nephron segments were stained with antibodies raised against the C-terminal peptide (anti-UTC) and N-terminal peptide (anti-UTN) of the UT. Treatment of the anti-UTC antibody with the UTN peptide did not affect immunoreactivity (C), while preabsorption with the UTC peptide resulted in complete extinction of the immunoreactive signals (arrows in D). Scale bar, 200 µm.