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Fig. 5. Effect of osmolality and Ca2+ on motility in (A) FWT and (B) SWT demembranated sperm. The demembranated sperm were suspended in reactivation solutions containing 220 µmol l-1 Mg-ATP2-, 75-650 mmol l-1 potassium acetate, 1 mmol l-1 free Mg2+, 1 mmol l-1 DTT, 0.5 mmol l-1 EGTA, 0.5 mmol l-1 EDTA, 20 mmol l-1 Hepes-NaOH (pH 8.0). Open triangles, 10-4 mol l-1 Ca2+; open squares, 10-7 mol l-1 Ca2+; filled circles, 10-8 mol l-1 Ca2+. Values are means ± S.D. (N=7).