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Fig. 4. Effect of (A) cAMP (10 µmol l-1), cGMP (10 µmol l-1), Ca2+ (10-4 mol l-1 free Ca2+) and reactivation solution (B) various concentrations of Ca2+ on reactivation of sperm motility in demembranated sperm. The demembranated sperm were suspended in reactivation solution containing 220 µmol l-1 Mg-ATP2-, 175 mmol l-1 potassium acetate, 1 mmol l-1 free Mg2+, 1 mmol l-1 DTT, 0.5 mmol l-1 EGTA, 0.5 mmol l-1 EDTA, 20 mmol l-1 Hepes-NaOH (pH 8.0). Values are means ± S.D. (N=5 in A; N=7 in B). *Significantly different (P<0.01).