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Fig. 2. (A-F) Immunolocalization of the Na+/K+-ATPase in Carcinus maenas. (A,B) Branchial cavity of zoea IV (cuticle detached from the branchiostegite epithelia). (C,D) Branchial cavity of megalopa. (E,F) Branchial cavity of crab I (cuticle detached from branchiostegite epithelia). (A,C,E) Fluorescent micrographs. (B,D,F) Corresponding phase-contrast pictures. Ag, anterior gill; bc, branchial chamber; brst, branchiostegite; cu, cuticle; gb, gill bud; gf, gill filament; pg, posterior gill. Bars, 50 µm.