Fig. 1. The effect of a diet low in arachidonic acid (low-ArA) and an ArA-enriched diet (high-ArA) in combination with acetylsalicylic acid (ASA) treatment on plasma cortisol responses to 5 min net confinement. Open circles, low-ArA; filled circles, low-ArA + ASA; open triangles, high-ArA; filled triangles, high-ArA + ASA. Samples were taken from non-stressed fish (t=0) and at several intervals after confinement (t=20 min, 60 min, 24 h). ASA-treated fish were subjected to confinement 4 h after the last dose of ASA. Each time point represents the mean value ± S.E.M. of 10 fish. The results of both graphs were combined for analysis and significant effects of the factors: ArA supplementation, ASA treatment, time after confinement, and/or interactions are indicated: ^*P<0.05, ^**P<0.01, ^***P<0.001 (3-way ANOVA).