Fig. 5. Analysis of Fxr1p isoforms in adult zebrafish tissues, Homogenates of adult zebrafish brain, muscle and testis were immunoblotted using Affi5 antibodies to detect the presence of molecular forms from Fxr1p. In brain a prominent band of approximately 73 kDa and a low-intensity band of approximately 70 kDa are present. In skeletal muscle high molecular mass isoforms of Fxr1p are present of approximately 86-88 kDa. The detection of Fxr1p isoforms in testis results in a prominent band of 73 kDa and weaker bands of 77 kDa and 88 kDa.