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Fig. 6. Single intact S. gregaria tubule. (A) Control tubules subjected to alkaline phosphatase protocol without chromogenic substrate; (B) as in A but with NBT/BCIP chromogenic substrate; (C) DAPI-stained; (D) high-magnification image of lower tubule, without chromogenic substrate; (E) high-magnification image of B. Scale bars, 100 µm.