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Fig. 3. Intact Ae. aegypti tubules. (A) Control tubules subjected to alkaline phosphatase staining protocol without chromogenic substrate; the junction with the gut is shown with an arrow; (B) as in A but subjected to alkaline phosphatase staining protocol in the presence of NBT/BCIP chromogenic substrate; the junction with the gut is shown with an arrow; (C) DAPI-stained; (D) as in C but also stained for alkaline phosphatase. Scale bars, 100 µm.