Fig. 2. The pattern of ubiquitin immunostaining in astrocytes changes after glucose deprivation (GD). Astrocyte cultures were subjected to sham wash (A,B) or allowed to recover 16 h after an 8 h GD insult (C–I). Uninjured cells show diffuse immunoreactivity with greater nuclear staining compared to cytoplasmic (A,B). After GD there is loss of nuclear staining and the appearance of aggregates throughout the cytoplasm, varying from coarse to fine. Scale bar, 10 µm.