Fig. 1. Ubiquitin staining patterns in CA1 neurons, 24 h following 8 min of dense forebrain ischemia. Ubiquitin staining is color-coded green, ß-gal staining is color-coded red. (A) A sham control animal not subjected to ischemia was examined 24 h later. A diffuse pattern in the processes (arrowheads) with strong nuclear staining (arrow) is observed. (B) In an animal subjected to dense forebrain ischemia, the pattern has changed to a patchy pattern in processes (arrowheads) with little nuclear staining (arrow). (C) An animal subjected to ischemia following injection with Herpes vector encoding only ß-gal and then colabeled for ß-gal to identify a vector targeted cell shows the same pattern in the targeted neuron as in B and in neighboring untargeted neurons with loss of nuclear staining (arrow). (D) An animal injected with Hsp-72 vector shows a relatively maintained pattern of ubiquitin staining in the neuron that is overexpressing Hsp72; note colocalization of ubiquitin and ß-gal staining in the nucleus (arrow) identified by the yellow color.