Fig. 8. (A) Cardiac myocytes were subjected to hypoxia–acidosis in the absence or presence of the mitochondrial permeability pore (MPTP) inhibitors bongrekic acid (BA) or decylubiquinone (DUB), as indicated. (B) Cardiac myocytes were exposed to normoxic or hypoxia–acidosis conditions. At the times indicated, cells were loaded with MitoTracker Red dye and analyzed by confocal microscopy as described (Kubasiak et al., 2002). Arrows indicate intense staining around nuclei in aerobic myocytes and reduced staining under hypoxia. Results are representative of three experiments.