Fig. 7. Characteristics of programmed death by BNIP3. (A) Cardiac myocytes were subjected to hypoxia–acidosis as described in Fig. 2. At the indicated times, samples of media were taken for analysis of lactate dehydrogenase (LDH) activity (open circles), or plates were stained with Trypan blue (filled circles) (Kubasiak et al., 2002). Data are expressed as % of cells stained with Trypan blue or % LDH released relative to total LDH in homogenates. (B) Cardiac myocytes were subjected to hypoxia–acidosis in the absence or presence of the broad-range caspase inhibitor Boc-D as indicated. Staurosporine (Sta; 0.1 µmol l^–1 for 8 h) is shown as a positive control.