Fig. 2. Western blot of total (T), soluble (S), and aggregated (A) proteins prepared with the addition of 0, 0.3, 0.5 mol l^–1 trehalose in extraction buffer. Flies were subjected to 4 h of anoxia and 5 µg of aggregated proteins were prepared by adding 0, 0.3, 0.5 mol l^–1 trehalose. Although the same amount of Na⁺/K⁺ATPase seemed to be present in total proteins, much less Na⁺/K⁺ATPase was present in the aggregated proteins with 0.5 mol l^–1 trehalose as compared with that lacking trehalose. Note that the beta-tubulin concentration did not change in each of T, S or A proteins as a function of trehalose.