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Fig. 3. (A) Northern blot hybridization of hypothalamic RNA probed with fowl AVT cDNA and GAPDH. Each lane was loaded with 20 µg of total cellular RNA. C, control; WD, water deprived; TP, testosterone propionate treated; TP+WD, testosterone propionate treated + water deprived for last 2 days of injection; EB, estradiol benzoate treated; EB+WD, estradiol benzoate treated + water deprived for last 2 days of injection. (B) Hypothalamic AVT mRNA. Values are means ± S.E.M. (N=10). *P<0.05; **P<0.01; ***P<0.001 (significance of difference from control).