Fig. 5. Western blot analysis of plasma membrane Ca²⁺-ATPase (PMCA3) in various crayfish tissues in intermolt (A), premolt (B) and postmolt (C). Total plasma membrane protein (30 µg) from gill, antennal gland, cardiac muscle and axial abdominal muscle was loaded in each lane. The membrane was hybridized to a polyclonal antibody (designed against amino acid residues 751-775 EGKEFNRRVRDESGGC of crayfish antennal gland PMCA3 deduced protein sequence; see Fig. 1A for location) and exposed to X-ray film for 2 min.