Fig. 4. Quantification of PMCA3 mRNA (A) and PMCA3 protein (B) from gill, antennal gland, cardiac muscle and axial muscle during molt stages. Values indicate the percentage expression difference compared with the intermolt (control values). Values were obtained from three different scanned X-ray film images and analyzed using KODAK 1D image analysis software. Values are means ± S.E.M. (N=3). All values, except premolt antennal gland, were significantly different (P<0.001) from their intermolt values. Statistical comparison (t-test) between postmolt and premolt expression is indicated as either not significant (NS, P>0.05) or significant (^*P<0.01; ^**P<0.001).