Fig. 1. The nucleotide and deduced amino acid sequences of land crab nitric oxide synthase (Gl-NOS) cDNA. The cDNA (3982 bp) contained a complete open reading frame (ORF) encoding a protein of 1199 amino acids (residue number indicated on the right). Locations and directions of degenerate primers used for nested PCR to obtain the initial cDNA are indicated by bold letters and solid arrowheads with broken lines. Locations and directions of sequence-specific forward primers and a degenerate reverse primer used for semi-nested PCR to obtain more of the 3' ORF are indicated by open arrowheads with broken lines. Solid arrowheads with solid lines indicate locations and directions of nested sequence-specific primers used for 5' and 3' RACE. The poly(A) signal (AATAAA) in the 3' UTR is boxed. GenBank accession #AY552549.