Fig. 2. Examples of H⁺ influx and efflux. 2-D vectors were calculated from measurements taken near the ovariole and 10 µm away in the x- and y-axis, and superimposed over an image of the ovariole. Vectors were classified as influx if the vector traveled over the ovariole image (left), or as efflux if the vector traveled away from the ovariole (right). Ion concentration gradients are shown as red gradients representing influx (left) or efflux (right).