Fig. 4. Quantification of S₂ myosin heavy chain (MHC) mRNA by real-time PCR in different lobster muscle samples (fiber bundles). Expression was highest in the superficial flexors and extensors (SF), followed by the S₁ fibers of the crusher claw closer (CR) and then by the fast fibers of the cutter claw closer (CT) and deep abdominal flexors (DA). Differences were analyzed by ANOVA of log-transformed values of copy number per µg of total RNA. A Bonferroni post-hoc test was used to determine which means were significantly different from one another (represented by different lowercase letters). Values are means ± S.D. (N=9-11 samples per group).