Fig. 2. Alignment of cDNA sequences encoding lobster muscle slow-twitch
(S1), fast and slow-tonic (S2) tropomyosin (Tm). Bases
that match at least one of the other sequences are shaded to indicate the
sequence identities among the three isoforms, and the stop codons (TAA) are
enclosed in a single box. The beginning of the start codon is indicated. The
S2 sequence shares 92% identity with the fast sequence within the
ORF, while the S1 and S2 sequences share 97% identity.
The S1 and S2 sequences are identical up to bp 833 of
the S2 sequence and are then divergent through the rest of the ORF
and 3' UTR. A common forward PCR primer was used to amplify both the
S1 and S2 sequence, while S1 reverse
(S1R) and S2 reverse (S2R) primers were
designed to isoform-specific sequences within the 3' UTR. The fast and
S1 Tm sequences have been published previously
(Mykles et al., 1998). The
GenBank accession numbers for the Tm sequences are AF034954 (fast), AF034953
(S1) and AY521627 (S2).