Fig. 1. Alignment of cDNA sequences encoding part of the open reading frame (ORF)
and 3' untranslated region (UTR) for the slow-twitch (S1),
fast and slow-tonic (S2) myosin heavy chains (MHC) to show sequence
similarities and the regions of the S1 and S2 sequences
amplified by PCR. Bases that match at least one of the other sequences are
shaded, and the stop codons (TAA) are enclosed in boxes. Each sequence shares
79% identity within the ORF. The 3' UTR follows the stop codon and
has low sequence identity. S1 forward (S1F),
S1 reverse (S1R), S2 forward (S2F)
and S2 reverse (S2R) MHC primer positions are indicated
by arrows adjacent to the S1 and S2 sequences,
respectively. Reverse PCR primers were designed to isoform-specific sequences
in the UTR, while forward primers were designed to anneal to regions of
relatively low sequence identity in the ORF. The fast and S1 MHC
sequences have been published previously
(Cotton and Mykles, 1993;
Medler and Mykles, 2003). The
GenBank accession numbers for the MHC sequences are U03091 (fast), AY232598
(S1) and AY521626 (S2).