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Fig. 3. Learning and memory of snails trained in 50 and 75 µmol l–1 of H2S. (A) A cohort of naïve snails (N=24) were trained using the same LTM training procedure as in Fig. 2 while being exposed to H2S at a concentration of 50 µmol l–1. This cohort of snails showed both learning and memory (ANOVA F(23,2)=23.9962 P<0.01; sessions 2 and 3 significantly different from session 1, P<0.01). (B) Another cohort of naïve snails (N=11) was subjected to the same LTM training procedure as in A except that these snails were exposed to an H2S concentration of 75 µmol l–1. Again both learning and memory occurred (ANOVA F(10,2)=6.6139, P<0.01; sessions 2 and 3 significantly different from session 1, P<0.05 and P<0.01, respectively).