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Fig. 1. Recordings of basolateral membrane potential (top traces) and intracellular activity (lower traces) of (A) K+ and (B) Cl– in unstimulated tubules. Basolateral membrane potential and ion activity were measured simultaneously using double-barrelled ion-selective microelectrodes (ISMEs). In this and subsequent figures, impalement is indicated by the upward-pointing arrows and the removal of electrode from the cell is indicated by downward-pointing arrows.