Fig. 6. Sections of 0 h (A–C), 1 h (D–F), 2 h (G–I) and 3 h (J–L) post-molt cuticle probed with antibodies raised against either the purified glycoprotein (A,D,G,J) or a 15-amino-acid peptide determined by Edman degradation of the purified material (B,E,H,K). Detection was by fluorescent-labeled secondary antibodies. Controls (C,F,I,L) lacking primary antibody show some autofluorescence in the epicuticle (arrowhead) and the hypodermal cells but none in the exocuticle (bracket). All photos were taken at the same light intensity and exposure time and were not digitally altered. Arrows indicate the interprismatic septa (IPS) staining less intensely at the later time periods. Scale bars, 50 µm.