Fig. 5. Coomassie Blue-stained gel (A) and corresponding immunoblot (B) of soluble proteins extracted at ecdysis and at various times immediately thereafter. The immunoblot was probed with antibodies raised against the Edman degradation-derived N-terminal amino acid sequence (anti-peptide). In both A and B, lane 1 contains 0 h extract, lane 2 contains 1 hextract, lane 3 contains 2 h extract, and lane 4 contains 3 h extract. MWS, molecular mass standards (kDa).