Fig. 4. An immunoblot experiment on unfractionated proteins from 0-h cuticle. Coomassie Blue staining of molecular mass standards (MWS) and cuticle extract (lane 1) are compared with immunoblots of the same extract probed with either anti-glycoprotein (lane 2) or anti-peptide (lane 3) antibodies. Lane 4 is an immunoblot of the purified glycoprotein probed with the anti-peptide antibody. Detection of the chemiluminescent product required 2 min exposure to film for the anti-glycoprotein blot (lane 2) and 30 s exposure for the anti-peptide (lanes 3 and 4).