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Fig. 3. Enzyme-treated purified glycoprotein subjected to SDS-PAGE and visualized by PAS staining of electroblotted membranes. (A) Lane 1, untreated glycoprotein; lane 2, chondroitinase-ABC treatment; lane 3, keratanase treatment; lane 4, heparinase II treatment. (B) Lane 1, untreated glycoprotein, lane 2, PNGase-F treatment. See Materials and methods for details.