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Fig. 2. Two glycoprotein purifications (A,B and C,D) from 0 h cuticle extracts performed identically yet yielded different results. The purified material retains one major Coomassie Blue-stained band but either one (A,B) or two (C,D) broad carbohydrate bands detectable by PAS staining. A and C are SDS-PAGE gels stained with Coomassie Blue for protein. B and D are PVDF membrane blots of similar gels stained with PAS for carbohydrate. In all cases, the first lane contains molecular mass standards (MWS), lane 1 is unfractionated 0 h cuticle protein extract, lane 2 is the Jacalin-positive fraction (Jac+) following lectin affinity chromatography, and lane 3 is the final purified product after gel filtration chromatography of the Jac+ fraction.