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Fig. 4. Serotonin levels in the silkmoth's protocerebrum and antennal lobe (AL) after application of different concentrations of serotonin (10-5 mol l-1, 10-4 mol l-1 and 10-3 mol l-1). 4 µl of serotonin were applied to the desheathed brain 3 min before measurement with HPLC. Values are means ± S.E.M. Sample size: control (N=18), 10-5 mol l-1 serotonin (N=18), 10-4 mol l-1 serotonin (N=25), 10-3 mol l-1 serotonin (N=26), wash for 10-4 mol l-1 serotonin (N=9), wash for 10-3 mol l-1 serotonin (N=9). Differences between bars marked with the same letters were not significant (Kruskal–Wallis test, P<0.05).