Fig. 5. Numbers of RFamide-ir neurons in the AL of M. sexta after hemolymph injection of 20E at P1 (A), P9 and P10/11 (B) and dissection several days later (see inset in Fig. 4). (A) Animals were injected with 20E at stage P1 and dissected 2 (P3) to 8/9 days (P9/10) later. Hormone-treated animals (open circles) showed a progressive increase in the numbers of RFamide-ir neurons compared to controls (filled circles) injected with saline. (B) Injections of 20E at P9 or P10/11 and dissection 6/7 days later (P9, open triangles) or 3/4–5/6 days later (P10/11, open circles) resulted in cell numbers that were not significantly different from the control animals (filled circles or triangles). Values are means ± S.D. The number of Als is shown for each stage. Inset in B shows wing pigmentation 6 days after injection of saline (control) or 20E at stage P10/11.