Fig. 2. Confocal images of 40 µm sections showing double immunostaining in the lateral cell group (LC) of P10 antennal lobes with antisera against RFamide (green, Cy2; Ai, Bi) and GABA (red, Cy3; Aiii, Biii). (Aii, Bii) Overlays of the respective immunostainings in Ai/Aiii and Bi/Biii. (A) RFamide-ir cell bodies in the LC, of which two show double labeling with the GABA antiserum (large arrows in Aii). The arrowhead (Ai) points to the large type II cell body, which like the root of the outer antenno-cerebral tract (small arrows) shows only RFamide and no GABA immunostaining. (B) In another section all type I cells colocalize RFamide and GABA immunoreactivity. Scale bars, 40 µm.