Fig. 1. Normarski images of 40 µm sections showing cell types I–V of the AL labeled with the RFamide antiserum. (A) Type I cells in the lateral cell group (LC) of a stage P15 AL. Staining within the glomeruli (GL) primarily results from type I neurons. No immunostaining is visible in the median cell group (MC). (B,C) Consecutive horizontal sections through a P13 AL. (B) In addition to glomerular staining, type I cell bodies give rise to stained fibers in the root of the outer antenno-cerebral tract (arrows). (C) Section ventral to B. The large cell body of the type II cell (arrow) has a posterior-medial position within the LC. Inset shows the primary neurite (arrows) leaving the AL without sending processes to the AL neuropil. (Di–iii) Type II–V neurons in a stage P1 pupa. Di and Dii are both superimposed images of two consecutive sections. Diii combines Di and Dii (total of four consecutive sections) with parts of the anterior laying cell groups omitted to better see the connections between cell bodies and neurites. The neurite (double arrow in Di) of the type II cell (large arrow in Dii, iii) leaves the AL towards the tritocerebrum (TC) while the neurite of the type V neuron (double arrowhead in Di) enters the AL. The neurite (arrowheads in Di) of the type IV neuron gives rise to processes that cover most of the developing AL area (solid outline in Di). The cell bodies of type II, III (arrowheads in Dii, iii), and IV neurons (small arrows in Dii, iii) are located anteriorly to the AL neuropil. Small arrow in Di, Dii points toward the connection site of the type IV neuron with its cell body. The type IV cell body lies in a cell group (broken outline in Dii), which is different from that of type II and III neurons (solid outline in Dii). A, anterior; D, dorsal; L, lateral; CN, coarse neuropil; MGC, male specific macroglomerular complex; TC, tritocerebrum. Scale bars, 100 µm (A–C); 50 µm (inset in B, Di–iii).