Fig. 6. Gyc-88E and Gyc-89Db expression in the central nervous system of third instar larvae. In situ hybridization was performed on isolated third-instar central nervous systems using the same riboprobes used in the embryo experiments. (A,B) Expression in the brain lobes. Gyc-88E (A) and Gyc-89Db (B) expression was found in scattered cells throughout the brain but was most prominent in a small cluster of cells located in the anterior medial region of each brain lobe (open arrowheads). (C–F) Expression in the ventral nerve cord. Gyc-88E (C,E) and Gyc-89Db (D,F) expression was found in scattered cells located laterally and in the midline (midline marked with broken line). The pair of images shown in C and E and in D and F are from the same preparation but are viewed at different focal planes. Anterior is up in all panels. Scale bar, 200 µm.