Fig. 5. Localization of Gyc-88E and Gyc-89Db expression in the central nervous system of Drosophila embryos. In situ hybridization experiments were performed on whole embryos using fragmented DIG-labeled Gyc-88E or Gyc-89Db riboprobes. (A,C) Gyc-88E expression in stage 16 embryos. Expression was detected in a segmental pattern in the ventral nerve cord and throughout the brain (open arrowhead). (B,D) Gyc-89Db expression in stage 17 embryos. A similar pattern of expression was detected in the ventral nerve cord and brain, although noticeably fewer cells stain in the brain with Gyc-89Db (open arrowhead). A and B show the horizontal view, while C and D show the lateral view. Application of a sense probe generated from Gyc-88E (E) or Gyc-89Db (F) demonstrates that the low level of ubiquitous background staining observed reflects non-specific hybridization. Anterior is always left and ventral is down in side views. Square brackets in C and D indicate the position of the ventral nerve cord. Scale bar, 100 µm.