Fig. 1. Western blot detection of ERK1 protein in the testicular extracts of R. esculenta at various times, 22°C and 12h:12h photoperiod. (A) Proteins (25 mg/lane) were resolved by SDS-PAGE, transferred to nitrocellulose membranes and then incubated with antibody raised against phospho-ERK1 and ERK1 protein. A specific band was observed of 44 kDa (determined by comparison with comigrating size markers). The blot is representative of three separate assays. (B) The amount of activated ERK1 was quantitated by using ImageQuant 5.2 Program and normalized by total ERK1. The values shown are representive of three separate experiments.