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Fig. 6. Opsonic activities are influenced by SpC3 and blocked by anti-SpC3-6H. Coelomic fluid (CF) was collected from sea urchins that had received two injections at 2-day intervals of LPS and was harvested 1 day after the second LPS injection. Yeast were opsonized for 40 min and phagocytosis was allowed to proceed for 45 min. Yeast were opsonized with either CF (yeast+CF), CF incubated for 2 h with anti-SpC3-6H (1:20 dilution; yeast+CF+{alpha}-SpC3-6H), CF incubated for 2 h with anti-profilin (1:20 dilution; yeast+CF+{alpha}-profilin) or ASW (yeast+ASW). Some of the yeast that had been opsonized with CF+anti-SpC3-6H were acid-washed prior to use in phagocytosis (yeast+CF+{alpha}-SpC3-6H washed). PSI, phagocytic stimulation index. Values are means ± S.E.M. (N=6). *P<0.05, yeast+CF+{alpha}-SpC3-6H vs. yeast+CF.