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Fig. 1. Map of South Florida and the Florida Keys depicting field locations in Florida Bay (FB-1) and Biscayne Bay (BB-1) where artificial habitats or shelters were deployed. The FB-1 site was used to document toadfish spawning activity and sample water for in situ nitrogen excretion within shelters. BB-1 was used to sample early life history stages of toadfish for OUC enzyme analysis and to harvest whole nests (shelter + adult + offspring) for experimental studies.