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Fig. 4. Electrophoretic separation of MHC isoforms in muscle samples and single fibres. (A) Two bands (MHC-1 and MHC-2A) can be seen in masseter (M), three bands (MHC-1 MHC-2A and MHC-2X) in diaphragm (D) and (MHC-1 MHC-2B and MHC-2X) in red semitendinosus (Sr), white semitendinosus (Sw) and longissimus dorsi (LD). (B) Two hybrid single fibres (2X-2B and 2X-2A) are compared with masseter (M), diaphragm (D) and longissimus (L). (C) Five bands (three fast MHC-2B, -2X and -2A, extraocular (Eo) and MHC-1, barely detectable) are separated in the extraocular rectus lateralis (RL) and two bands (MHC-2X and MHC-2B) in rectractor bulbi (RB). Three single fibres (pure 2B and hybrid 2X-2B and 2X-2A) are also shown.