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Fig. 1. Comparison of the reproductive activity of control versus rapidly cold-hardened (RCH) D. melanogaster tested at 16°C. Immediately prior to the mating trial, RCH pairs (N=20) were acclimated to 16°C for 2 h, while control pairs (N=22) were transferred from 23°C to 16°C immediately prior to the 1-h observation period. Asterisks denote significant differences from control ({chi}2, P<0.05).