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Fig. 3. Semiquantitative RT-PCR analysis of V-type H+-ATPase (VHA) mRNA abundance in sternal epithelial cells of Porcellio scaber during the mineralization–demineralization cycle. (A) Demonstration of template-dependent quantification of VHA B-subunit mRNA. (B) Relative expression of PMCA in the anterior (ASE) and posterior sternal epithelium (PSE).