Fig. 3. Chloride cell (CC) number and size determined by LSC analysis from gill epithelial cell suspensions obtained after 10 days of acute and gradual acclimation from FW to SW. (A) CC number does not increase significantly after acclimation to 1x SW or 2.4x SW (P>0.05 in each case). (B) CC volume increases significantly after acclimation to 1x and 2.4x SW (P<0.05; ^aFW vs. 1x SW, ^bFW vs. 2.4x SW, ^c1x SW vs. 2.4x SW). N=4 animals for each salinity and time.