Fig. 2. (A-C) Laser scanning cytometry (LSC) analysis of chloride cells (CC) from killifish acclimated for 10 days to FW (blue), 1x SW (green) and 2.4x SW (red). CC were identified based on DASPMI fluorescence with the LSC. CC area and maximal intensity of DASPMI fluorescence were recorded for each CC during scanning by WinCyte software and plotted as scattergrams. (A) FW; (B) 1x SW; (C) 2.4x SW. The boxes enclose all accepted data and the circles enclose events that were used for generating image galleries with the LSC relocation function. (D-F) Additional scattergrams showing the relationship between CC area and integrated DASPMI fluorescence per CC were also recorded. (D) FW; (E) 1x SW; (F) 2.4x SW. CCs have higher DASPMI fluorescence than other cell types and are colored in all panels.