Fig. 4. Identification of dynamin in Paramecium cells using antibodies against the C-terminal region of human dynamin 2. (A) SDS-PAGE (Ponceau Red stained) and (B) western blot analysis of protein fraction S2 (lane 1). The recombinant rat dynamin 2 (lane 2) was used as a positive control for immunoblot analysis. One immunoreactive band of 105 kDa was detected. Positions of the molecular marker are shown on the left. The western blots shown are representative of three independent experiments.